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Abstract (in English)
Secondary metabolites of fungi have low molecular weight which play a significant role in agriculture during pre- and post-harvest stages. Alternaria is one of the fungi that produce toxins and have a major impact on plant pathogenesis. The aim of this study was to find secondary metabolites from Alternaria fungi on tomato plants. In this study, Alternaria spp. was isolated from tomato fields in the Jalriz district of Maidan Wardak province, Afghanistan. After isolation and purification, the fungus was cultured on wheat seeds to stimulate secondary metabolite production. Following 21 days of cultivation, ethyl acetate was employed to extract the secondary metabolites, which were subsequently dissolved in N-hexane and analyzed using gas chromatography-mass spectrometry (GC-MS). The GC-MS analysis revealed the presence of thirteen chemical compounds, including Tetradecane 2,6,10-trimethyl, Heptadecane 2,6,10,15 tetramethyl, Heptacosane, Docosane, Heneicosane, Bis(2-ethylhexyl) phthalate, Phenol 2,4-bis(1,1 dimethylethyl) phosphite (3:1), Cardamonin bis(tert-butyldimethylsilyl) ether, N-Hexadecanoic acid, 1-Hexadecanol 2-methyl, Squalene, Eicosane, and 3-(Benzylthio) acrylic acid, methyl ester. All the identified compounds from Alternaria exhibit varying effects on plants at different stages of their life cycle.