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Abstract (in English)
The morel mushroom (Morchella spp.) is one of the most valuable edible mushrooms worldwide due to its high nutritional, medicinal, and economic value. Despite its importance, in Afghanistan the use of morels is mostly limited to wild collection, and scientific cultivation techniques remain poorly developed. In this study, a local variety of morel mushroom was collected from Andarab District of Baghlan Province and examined in the biotechnology laboratory of Kabul University. Tissue culture was carried out in five Petri dishes for seven days in an incubator under a Completely Randomized Design (CRD) using PDA (Potato Dextrose Agar) medium. The produced spawn was then inoculated onto different compost substrates. The results showed that tissue culture and spawn production were successfully achieved under laboratory conditions. However, inoculation of spawn on different compost substrates did not result in fruiting body formation after 60 days of incubation.